TaqL and MspL RFLPs are detected by the human 2,3-biphosphoglycerate mutase (BPGM) cDNA.

Source/Description: The XMEL4 clone is a 1.5 kb cDNA subcloned into the EcoRI site of pBluescript SKII +. XMEIA was isolated from a human melanoma cDNA library. Sequence analysis showed complete homology between XMEL4 and the 2,3-biphosphoglycerate mutase (BPGM) cDNA sequence (1, 2). Polymorphism: Both Taql and Mspl detect two-allele polymorphisms when probed with XMEIA Frequency: Allele frequencies were determined by typing the 80 parents of the CEPH reference families. Enzyme Taql Mspl Allele 1 2 1 2 Size 6.0 kb 5.5 kb 7.0 kb 6.2 kb Frequency 0.55 0.45 0.83 0.17